Our sensor assay enables parallel functional evaluation of ~20,000 RNAi triggers, selecting the most potent ones.
We only use single-copy integration, avoiding miRNA saturation and reducing off-targeting.
Doxycycline induces gene knockdown. Withdrawal of doxycycline results in pre-knockdown levels of expression.
Fast and cost-effective
Our engineering strategies decreased the timelines of animal model generation from years to <6 months.
Multiple gene silencing
RNAi can be employed to simultaneously silence the expression of two or more genes
Mirimus has fused together unparalleled expertise of RNA interference technology and genetic engineering of mouse models to create advanced platforms for rapid and cost-effective production of customized RNAi mouse models with potent, reversible gene silencing.
Activation of the Wnt pathway, often through deregulation of APC, is one of the most frequent events in human cancers. To model this event in mice, we inducibly silenced Apc using RNAi. After 4-6 weeks of doxycycline treatment, all mice developed leukemia/lymphomas, which were transplanted to secondary recipients. Shown in the video is one animal that presented with lymphoma associated hind limb paralysis at 4 weeks post transplantation. After 3 days following doxycycline withdrawal, the mouse regained full mobility. Our shAPC RNAi mice enabled us for the first time to explore the role of APC suppression and its continued deregulation in tumor maintenance.
